Neurobehavioral and Micro Structural Evaluation of the Anti-anxiety Potential of Flaxseed oil Following Rotenone-induced Parkinson’ Disease in Mice
Philemon Dauda Shallie *
Department of Anatomy, Olabisi Onabanjo University, Nigeria
Bamidele Richard Babatunde
Department of Anatomy, Olabisi Onabanjo University, Nigeria
Olugbenga Olawole Olayinka
Department of Anatomy, Olabisi Onabanjo University, Nigeria
Damilola Joseph Talabi
Department of Anatomy, Olabisi Onabanjo University, Nigeria
Helen Bassey Akpan
Department of Anatomy, Olabisi Onabanjo University, Nigeria
Royhaan O. Folarin
Department of Anatomy, Olabisi Onabanjo University, Nigeria
Oluwole Ojo Alese
Department of Anatomy, Ekiti State University, Nigeria
Oluwadamilola Faith Shallie
Department of Anatomy, Olabisi Onabanjo University, Nigeria
*Author to whom correspondence should be addressed.
Abstract
Aim: This study investigated the neurobehavioral and micro structural anti-anxiety potential of flaxseed oil following rotenone-induced Parkinson’ disease [PD] in mice
Study Design: Fifty-six adult male and female mice (Mus musculus) weighing between 23.9-26.3 grams were used for this study. The mice were randomly placed into four groups of fourteen mice each: A (Control; mice pellets), B (Rotenone 3 mg/kg, IP), C (Rotenone + Flaxseed oil 0.3 ml orally), and D (0.3 ml Flaxseed + Rotenone).
Place and Duration of the Study: Department of Anatomy, Olabisi Onabanjo University. Between May and September, 2016.
Methodology: The brains were excised, weighed and appropriate sections taken and processed histology and labelled with H&E, silver and Nissl stains and immuno-cytochemically with GFAP.
Results: The results showed significant (P<0.005) increase in anxiety related activities and neuronal structural derangement, and degeneration of astrocytes in the rotenone-induced Parkinson’s mice, which were counter/ameliorated by flaxseed oil treatments.
Conclusion: In conclusion, flaxseed oil acts as a neuro-protective agent against the insult of rotenone model of Parkinson’s disease, thus it should be further evaluated as a potential therapeutic candidate in the management/treatment of Parkinson's disease.
Keywords: Parkinson’s disease, flaxseed oil, hippocampus, anxiety, rotenone